Reversed-Phase Chromatography of Escherichia Coli Ribosomal Proteins. Correlation of Retention Time With Chain Length and Hydrophobicity

Document Type

Article

Publication Date

11-28-1990

Description

The elution times of 52 bacterial ribosomal proteins from a C4 reversed-phase column have been predicted. The prediction is based upon the use of the hydrophobicity coefficients for the protein amino acid content as defined by Guo [J. Chromatogr., 359 (1986) 499-518]. A strong positive correlation was observed when the difference between predicted and observed protein retention time was plotted against the product of net hydrophobicity and natural log of protein chain length. Observations with this class of related proteins strengthens and extends the observations of Mant [J. Chromatogr., 476 (1989) 363-375]. Observed deviations from predicted chromatographic behavior can be explained for several proteins which elute as dimers or which have modified amino acid residues.

Share

COinS