Protein Kinase C-Mediated Contractile Response of the Rat Vas Deferens
Document Type
Article
Publication Date
8-6-1992
Description
The role of protein kinase C (PKC) in mediating contractile responses in the rat vas deferens was studied. Phorbol-12,13-di-acetate (PDA) in the presence of 20 mM K+ elicited a concentration-dependent response with an EC50 of 190 nM. The non-PKC activator 4α-phorbol (2 μM) was unable to elicit contraction in 20 mM K+ buffer. Incubation of rat vas deferens with the PKC inhibitor iso-H7 (30 μM) attenuated the response to norepinephrine (NE) ane neurokinin A, with maximal effects depressed to 42 and 39% of control, respectively. Responses to 60 mM K+ and 2 μM PDA (20 mM K+) was also significantly inhibited by iso-H7. In the presence of 2 μM PDA and 20 mM K+, the NE concentration-effect curve was shifted 3,6-fold to the right of the control curve in a parallel manner. 4α-Phorbol (20 mM K+) at the same concentration did not produce this effect. These results suggest a significant role for PKC in the contractile response of the rat vas deferens.
Citation Information
Abraham, S. T.; and Rice, Peter J.. 1992. Protein Kinase C-Mediated Contractile Response of the Rat Vas Deferens. European Journal of Pharmacology. Vol.218(2-3). 243-249. https://doi.org/10.1016/0014-2999(92)90175-4 PMID: 1330595 ISSN: 0014-2999