Growth of Morris Hepatoma No. 7794A With and Without Vitamin B6. Effect of Inoculation Time
Document Type
Article
Publication Date
1-1-1976
Description
The effect of time of inoculation of tumor cells on the growth of the highly differentiated transplantable Morris hepatoma 7794A under vitamin B6 deficient and control conditions was studied. Groups of weaning Buffalo strain rats were fed ad libitum a diet lacking pyridoxine or were pair-fed the same diet supplemented with the vitamin. They were then inoculated intramuscularly in both hind legs with 7794A hepatoma cells at the beginning (to) and after 7, 15, and 31 days on their respective diets. Animals were sacrificed 39-49 days after inoculation, tumors were excised and after removal of connective tissue they were weighed. The average weight of hepatomas grown in animals fed the vitamin deficient diet for the same length of time was always similar and in all cases was less than the average weight of those hepatomas grown in pair-fed (control) animals. The difference between average tumor weights was not statistically significant (P < 0·5) except when inoculation was performed after 31 days. The average weight of tumors from pair-fed controls, in this case, was 3 times higher over that of tumors grown in animals fed the vitamin deficient diet. The significance level was P < 0·05. The data are interpreted to show that caloric restriction (pair-feeding) allows hepatoma 7794A to grow as during ad libitum conditions provided inoculation takes place after 31 days of feeding period.
Citation Information
Tryfiates, George P.; and Morris, H. P.. 1976. Growth of Morris Hepatoma No. 7794A With and Without Vitamin B6. Effect of Inoculation Time. European Journal of Cancer (1965). Vol.12(1). 9-12. https://doi.org/10.1016/0014-2964(76)90117-1 PMID: 178510 ISSN: 0014-2964