Mutagenic Activity Associated with Cooling Tower Waters Treated with a Biocide Containing 5-Chloro-2-methyl-4-isothiazolin-3-one
Document Type
Article
Publication Date
1-1-1987
Description
With the Ames Salmonella-mamMalian microsome test, significant mutagenic activity was detected in cooling tower water shortly (same day) after treatment with a biocide (CL2150) containing 5-chloro-2-methyl-4-isothiazolin-3-one (5-chloro-IT). Dose-related mutagenic responses with TA97 (-S9) and TA100 (-S9) were produced with the acid fraction (extracted at pH <2 with methylene chloride) of this cooling water sample (specific mutagenic activities of 281000 and 118000 net revertants/L equiv of water, respectively). This mutagenic activity detected in cooling water sampled in mid-summer did not persist beyond the first day of CL2150 treatment. The mutagenic activity displayed by the cooling waters with TA97 (-S9) exceeded that associated with the extractable 5-chloro-IT concentration (determined by capillary gas chromatography-mass spectrometry).
Citation Information
Woodall, George M.; Pancorbo, Oscar C.; Blevins, R. Dean; and Ferslew, Kenneth E.. 1987. Mutagenic Activity Associated with Cooling Tower Waters Treated with a Biocide Containing 5-Chloro-2-methyl-4-isothiazolin-3-one. Environmental Science and Technology. Vol.21(8). 815-820. https://doi.org/10.1021/es00162a014 ISSN: 0013-936X