Identification of Phosphate Substitution Sites by NMR Spectroscopy in a Water-Soluble Phosphorylated (1→3)-β-D-Glucan
Document Type
Note
Publication Date
1-1-1998
Description
Detailed analysis of the site-specific phosphorylation of a glucan phosphate from Saccharomyces cerevesiae has been carried out by 2D NMR techniques. Phosphorylation has been shown to be limited to the C-6 and C-2 positions, with the C-6 resonance showing two slightly different environments. Phosphorylation at C-4 is hindered due to proximity effects with the neighboring glucose ring oxygen. Noncovalently bound, nondialyzable phosphate appears to be coordinated to the nonphosphorylated HO-2 group of the helical polymer.
Citation Information
Lowman, Douglas; Ensley, Harry; and Williams, David. 1998. Identification of Phosphate Substitution Sites by NMR Spectroscopy in a Water-Soluble Phosphorylated (1→3)-β-D-Glucan. Carbohydrate Research. Vol.306(4). 559-562. https://doi.org/10.1016/S0008-6215(98)00014-7 ISSN: 0008-6215