Perturbation of Lipid Metabolism in L-M Cultured Cells by Elaidic Acid Supplementation: Formation of Fatty Alcohols
Document Type
Article
Publication Date
12-28-1979
Description
Supplementation of culture medium with elaidic acid (400 μg/flask) in L-M cells results in the formation of an otherwise undetected lipid component. We have identified this lipid component to be a mixture of free fatty alcohols containing primarily elaidyl alcohol with cetyl, stearoyl, and oleoyl alcohols as minor constituents. Formation of fatty alcohols by fatty acid supplementation seems to be specific with trans fatty acids (i.e., elaidate, trans vaccenate, and linolelaidate); addition of stearate and oleate to the L-M cells does not produce fatty alcohols. The fatty alcohols accumulated by the trans fatty acid supplementation are associated with both the particulate and supernatant fractions of the cells.
Citation Information
Lee, Ten C.; Hougland, Arthur E.; and Stephens, Nelson. 1979. Perturbation of Lipid Metabolism in L-M Cultured Cells by Elaidic Acid Supplementation: Formation of Fatty Alcohols. Biochemical and Biophysical Research Communications. Vol.91(4). 1497-1503. https://doi.org/10.1016/0006-291X(79)91234-8 PMID: 526318 ISSN: 0006-291X