Perturbation of Lipid Metabolism in L-M Cultured Cells by Elaidic Acid Supplementation: Formation of Fatty Alcohols

Document Type

Article

Publication Date

12-28-1979

Description

Supplementation of culture medium with elaidic acid (400 μg/flask) in L-M cells results in the formation of an otherwise undetected lipid component. We have identified this lipid component to be a mixture of free fatty alcohols containing primarily elaidyl alcohol with cetyl, stearoyl, and oleoyl alcohols as minor constituents. Formation of fatty alcohols by fatty acid supplementation seems to be specific with trans fatty acids (i.e., elaidate, trans vaccenate, and linolelaidate); addition of stearate and oleate to the L-M cells does not produce fatty alcohols. The fatty alcohols accumulated by the trans fatty acid supplementation are associated with both the particulate and supernatant fractions of the cells.

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