Chemical Cross-Linking and Its Effect on Fatty Acid Synthetase Activity in Intact Chloroplasts From Euglena gracilis
Document Type
Article
Publication Date
12-16-1988
Description
Intact chloroplasts were isolated from Euglena gracilis variety bacillaris, and aliquots were exposed to several different chemical cross-linking reagents. The reagents penetrated the triple membrane of Euglena chloroplasts. This was shown by gradient acrylamide gel electrophoresis under denaturing conditions. The activity of the nonaggregated fatty acid synthetase of Euglena was located within the chloroplast stroma, and the effects of dimethylsuberimidate cross-linking on the activity of the enzyme system were examined. The acyl-carrier protein concentration in the chloroplast was measured at about 0.24 mM.
Citation Information
Worsham, Lesa M.; Tucker, Margie M.; and Lou Ernst-Fonberg, Mary. 1988. Chemical Cross-Linking and Its Effect on Fatty Acid Synthetase Activity in Intact Chloroplasts From Euglena gracilis. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. Vol.963(3). 423-428. https://doi.org/10.1016/0005-2760(88)90310-4 PMID: 3143414 ISSN: 0005-2760