Behavior of Acyl Carrier Proteins on Western Blots

Creator(s)

Lesa M. Worsham, Quillen-Dishner College of Medicine
Margie Tucker, Quillen-Dishner College of Medicine
M. Lou Ernst-Fonberg, Quillen-Dishner College of Medicine

Document Type

Article

Publication Date

4-2-1990

Description

Acyl carrier proteins (ACPs) from Escherichia coli and Euglena were analyzed on Western blots using rabbit antibodies raised against E. coli ACP. Euglena ACP, unlike that from E. coli, behaves upon electrophoresis under denaturing conditions as its size would predict. Oligomeric forms of both ACPs were evident on Western blots, but the bacterial ACP had more tendency to aggregate. That the oligomeric forms were not due to impurities was shown by their regeneration from low-Mr protein, reaction with antibodies isolated from Iow-Mr protein, and by molecular weight determination of the ACP by low-angle laser light scattering.

Citation Information

Worsham, Lesa M.; Tucker, Margie; and Lou Ernst-Fonberg, M.. 1990. Behavior of Acyl Carrier Proteins on Western Blots. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. Vol.1043(2). 198-202. https://doi.org/10.1016/0005-2760(90)90296-A PMID: 2107880 ISSN: 0005-2760