Reductive Methods for Isotopic Labeling of Antibiotics
Document Type
Article
Publication Date
8-15-1989
Description
Methods for the reductive methylation of the amino groups of eight different antibiotics using 3HCOH or H14COH are presented. The reductive labeling of an additional seven antibiotics by NaB3H4 is also described. The specific activity of the methyl-labeled drugs was determined by a phosphocellulose paper binding assay. Two quantitative assays for these compounds based on the reactivity of the antibiotic amino groups with fluorescamine and of the aldehyde and ketone groups with 2,4-dinitrophenylhydrazine are also presented. Data on the cellular uptake and ribosome binding of these labeled compounds are also presented.
Citation Information
Champney, W. S.. 1989. Reductive Methods for Isotopic Labeling of Antibiotics. Analytical Biochemistry. Vol.181(1). 90-95. https://doi.org/10.1016/0003-2697(89)90398-9 PMID: 2817384 ISSN: 0003-2697