Orthopoxvirus Gene Expression in Xenopus Laevis Oocytes: A Component of the Virion Is Needed for Late Gene Expression
Document Type
Article
Publication Date
1-1-1990
Description
We have examined the feasibility of using Xenopus laevis oocytes microinjected with rabbit poxvirus as a system to study poxvirus gene expression. The injection of either intact virus or subviral cores resulted in accurate synthesis of viral proteins. This expression was dependent on the multiplicity of injected virus, with the optimal injected dose being equivalent to approximately 300 PFU per oocyte. Extensive viral gene expression including late viral protein synthesis was observed when intact virions were microinjectd into the oocyte. However, the injection of subviral cores resulted in only early protein synthesis. When oocytes were injected with a mixture of subviral cores and the nonionic detergent-soluble fraction was removed from virus during the preparation of cores, both early and late viral proteins were synthesized. Therefore, the detergent-soluble fraction appears to contain a factor(s) required for the transition from early to late gene expression.
Citation Information
Massung, R. F.; and Moyer, R. W.. 1990. Orthopoxvirus Gene Expression in Xenopus Laevis Oocytes: A Component of the Virion Is Needed for Late Gene Expression. Journal of Virology. Vol.64(5). 2280-2289. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC249389/ PMID: 2157885 ISSN: 0022-538X