Ozone Inactivation of Human α1-Proteinase Inhibitor
Document Type
Article
Publication Date
1-1-1980
Description
Ozone decreased the trypsin, chymotrypsin, and elastase inhibitory activities of human α1-proteinase inhibitor (α1-PI) both in plasma and in solutions of the pure inhibitor. The total loss of porcine elastase inhibitory activity required 18 mol of ozone/mol of pure α1-PI and approximately 850 mol of ozone/mol of α1-PI in plasma. A corresponding loss of the ability to inhibit human leukocyte elastase was observed. Inactivated α1-PI contains four residues of methionine sulfoxide, in addition to oxidized tyrosine and tryptophan. Electrophoretic analysis demonstrated that the ozone-inactivated α1-PI did not form normal complexes with serine proteinases. These findings suggest that the inhalation of ozone could inactivate α1-PI on the airspace side of the lung to create a localized α1-PI deficiency, which might contribute to the development of emphysema.
Citation Information
Johnson, D. A.. 1980. Ozone Inactivation of Human α1-Proteinase Inhibitor. American Review of Respiratory Disease. Vol.121(6). 1031-1038. https://www.atsjournals.org/doi/10.1164/arrd.1980.121.6.1031?url_ver=Z39.88-2003andrfr_id=ori:rid:crossref.organdrfr_dat=cr_pub%20%200pubmed PMID: 6903114 ISSN: 0003-0805