Impaired T Cell-Mediated Macrophage Activation in CD40 Ligand-Deficient Mice
Document Type
Article
Publication Date
1-1-1996
Description
The expression of the ligand for CD40 (CD40L) is critical for induction of T cell-dependent Ab responses. To examine how critical the expression of CD40L is for induction of cell-mediated immune responses, the ability of T cells from CD40L knockout mice to activate macrophage effector function was assessed. CD4+ T cells from CD40L-knockout mice were fourfold less effective than +/+ T cells in activating the nitric oxide response in allogeneic macrophages. CD40L-knockout T cells that were fixed with paraformaldehyde after a 6-h activation period, a time point at which CD40L dominates the macrophage-activating capability of the T cell, could activate neither macrophage production of inflammatory cytokines (TNF-α) nor generation of reactive nitrogen intermediates. After 24 h of activation, however, both CD40L-knockout and +/+ T cells could induce similar but weak responses from the macrophages. This study demonstrates that animals deficient in CD40L expression display a deficiency in T cell-dependent macrophage-mediated immune responses.
Citation Information
Stout, Robert D.; Suttles, Jill; Xu, Jianchao; Grewal, Iqbal S.; and Flavell, Richard A.. 1996. Impaired T Cell-Mediated Macrophage Activation in CD40 Ligand-Deficient Mice. Journal of Immunology. Vol.156(1). 8-11. https://pubmed.ncbi.nlm.nih.gov/8598498/ PMID: 8598498 ISSN: 0022-1767