Erratum to: MiR-9-5p Down-Regulates PiT2, but not PiT1 in Human Embryonic Kidney 293 Cells (J Mol Neurosci, 10.1007/s12031-017-0906-0)
Document Type
Erratum
Publication Date
5-1-2017
Description
The original version of this article unfortunately contained mistakes in the Author group and Abstract sections. Author D. P. Bezerra’s name was incorrectly presented as “D. P. Paiva”. This abstract version substitutes the previous one: Abstract PiT1 (SLC20A1) and PiT2 (SLC20A2) are members of the mammalian type-III inorganic phosphate transporters and recent studies linked SLC20A2 mutations with primary brain calcifications. MicroRNAs (miRNAs) are endogenous noncoding regulatory RNAs and MicroRNA-9 (miR-9) modulates neurogenesis but is also involved with different types of cancer. We evaluated possible interactions between miR-9 and the phosphate transporters (PiT1 and PiT2). SLC20A2, platelet-derived growth factor receptor beta (PDGFRB) and Fibrillin-2 (FBN2) showed binding sites with high affinity for mir-9, In silico. miR-9 mimic was transfected into HEK293 cells and expression was confirmed by RT-qPCR. Overexpression of miR-9 in these cells caused a significant reduction in PiT2 and FBN2. PDGFRB appeared to be decreased, but was not significantly down-regulated. PiT1 showed no significant difference relative to controls. The down-regulation of PiT2 protein by miR-9 was confirmed by western blotting. In conclusion, we showed that miR-9 can down-regulate PiT2, in HEK293 cells.
Citation Information
Bezerra, D. P.; Keasey, M.; and Oliveira, J. R.M.. 2017. Erratum to: MiR-9-5p Down-Regulates PiT2, but not PiT1 in Human Embryonic Kidney 293 Cells (J Mol Neurosci, 10.1007/s12031-017-0906-0). Journal of Molecular Neuroscience. Vol.62(1). 34. https://doi.org/10.1007/s12031-017-0913-1 PMID: 28401368 ISSN: 0895-8696