Document Type
Article
Publication Date
5-11-2017
Description
Nuclear factor kappa B (NFκB) is an inflammatory transcription factor that plays an important role in the host immune response to infection. The potential for chlamydiae to activate NFκB has been an area of interest, however most work has focused on chlamydiae impacting human health. Given that inflammation characteristic of chlamydial infection may be associated with severe disease outcomes or contribute to poor overall fitness in farmed animals, we evaluated the ability of porcine chlamydiae to induce NFκB activation in vitro. C. pecorum infection induced both NFκB nuclear translocation and activation at 2 hours post infection (hpi), an effect strongly enhanced by suppression of host de novo protein synthesis. C. suis and C. trachomatis showed less capacity for NFκB activation compared to C. pecorum, suggesting a species-specific variation in NFκB activation. At 24 hpi, C. pecorum induced significant NFκB activation, an effect not abolished by penicillin (beta lactam)-induced chlamydial stress. C. pecorum-dependent secretion of interleukin 6 was also detected in the culture supernatant of infected cells at 24 hpi, and this effect, too, was unchanged by penicillin-induced chlamydial stress. Taken together, these results suggest that NFκB participates in the early inflammatory response to C. pecorum and that stressed chlamydiae can promote inflammation.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Leonard, Cory A.; Schoborg, Robert V.; and Borel, Nicole. 2017. Productive and Penicillin-Stressed Chlamydia Pecorum Infection Induces Nuclear Factor Kappa B Activation and Interleukin-6 Secretion in Vitro. Frontiers in Cellular and Infection Microbiology. Vol.7(MAY). https://doi.org/10.3389/fcimb.2017.00180 PMID: 28553623
Copyright Statement
2017 Leonard, Schoborg and Borel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.