Document Type
Article
Publication Date
12-7-2018
Description
Glypican-3 (GPC3), the cellular membrane proteoglycan, has been established as a tumor biomarker for early diagnosis of hepatocellular carcinoma (HCC). GPC3 is highly expressed in more than 70% HCC tissues detected by antibody-based histopathological systems. Recently, aptamers, a short single-strand DNA or RNA generated from systematic evolution of ligands by exponential enrichment (SELEX), were reported as potential alternatives in tumor-targeted imaging and diagnosis. In this study, a total of 19 GPC3-bound aptamers were successfully screened by capillary electrophoresis (CE)-SELEX technology. After truncated, AP613-1 was confirmed to specifically target GPC3 with a dissociation constant (KD) of 59.85 nM. When modified with a phosphorothioate linkage, APS613-1 targeted GPC3 with a KD of 15.48 nM and could be used as a specific probe in living Huh7 and PLC/PRF/5 imaging, GPC3-positive cell lines, but not in L02 or A549, two GPC3-negative cell lines. More importantly, Alexa Fluor 750-conjugated APS613-1 could be used as a fluorescent probe to subcutaneous HCC imaging in xenograft nude mice. Our results indicated that modified AP613-1, especially APS613-1, was a potential agent in GPC3-positive tumor imaging for HCC early diagnosis.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Citation Information
Dong, Lili; Zhou, Hongxin; Zhao, Menglong; Gao, Xinghui; Liu, Yang; Liu, Dongli; Guo, Wei; Hu, Hongwei; Xie, Qian; Fan, Jia; Lin, Jiang; and Wu, Weizhong. 2018. Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 When Used for Hepatocellular Carcinoma Cell Imaging. Molecular Therapy - Nucleic Acids. Vol.13 376-386. https://doi.org/10.1016/j.omtn.2018.09.013
Copyright Statement
2018 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).