Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
8-2023
Committee Chair or Co-Chairs
Abbas Shilabin
Committee Members
Aleksey Vasiliev, Sean Fox
Abstract
As antimicrobial resistance persistently disrupts the treatment of microbial infection, natural product chemistry has played a significant role in identifying novel drugs with novel modes of action critical to getting ahead of resistance. The primary goal of this project is to extract and identify potential novel antimicrobial natural products produced by Arthrobacter sp. Although underexplored, Arthrobacter sps. have been known to produce bioactive compounds with versatility; one such is a depsipeptide with quorum-sensing inhibitory activity.1 In this research, an Arthrobacter sp. TAJX1902, isolated from a soil sample, showed inhibitory activity against a filamentous indicator bacterium and a violacein-producing Janthinobacterium sp. Arthrobacter sp. TAJX1902 was cultured using rich medium broth and agar to extract and isolate metabolites. Isolated compounds were characterized via spectroscopic techniques, including 1D and 2D-NMR spectroscopy and GCMS analysis. Arthrobacter sp. TAJX1902 produces Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl), and five other bioactive cyclic dipeptides (CDPs).
Document Type
Thesis - embargo
Recommended Citation
Arije, Amonah, "Isolation and Structural Determination of Bioactive Metabolites from the Soil Bacterium, Arthrobacter sp. TAJX1902" (2023). Electronic Theses and Dissertations. Paper 4261. https://dc.etsu.edu/etd/4261
Copyright
Copyright by the authors.