Location
D.P. Culp Center Ballroom
Start Date
4-5-2024 9:00 AM
End Date
4-5-2024 11:30 AM
Poster Number
19
Name of Project's Faculty Sponsor
Ashana Puri
Faculty Sponsor's Department
Pharmaceutical Sciences
Competition Type
Competitive
Type
Poster Presentation
Presentation Category
Health
Abstract or Artist's Statement
Atopic dermatitis, commonly known as eczema, is a chronic inflammatory disorder caused by an overactive immune system. Atopic dermatitis usually develops in early childhood and is prevalent in people with family history of eczema. Several studies have suggested the use of cromolyn sodium (CS), a mast cell stabilizer, as an effective therapeutic agent for treating atopic dermatitis. This study explored the effect of the use of sodium caprylate and sodium oleate as enhancers on topical delivery of CS from a gel-based formulation. An extemporaneously prepared formulation called the Magic Masto Lotion, MML (0.3% CS), has been making waves in terms of the incredible results the lotion offers in the treatment of systemic mastocytosis. Since there is no past literature validating the effectiveness of this MML, another study was established to compare the topical in vitro delivery of CS from MML with the formulated gels. In this study, three separate gels were made by combining CS (4% w/w) with sodium oleate, sodium caprylate and without any enhancer. Drug and enhancers were in the molar ratio of 1:2. Hydroxypropyl cellulose (1.5% w/w) was used as a gelling agent, and respective amounts of propylene glycol as base for the gels. The recipe for the MML was also replicated with CS (0.3% w/w). In vitro permeation studies (24 h) were performed for all formulations using porcine ear skin mounted on Franz diffusion cells maintained at 37°C. Drug content in both the receptor and skin samples were analyzed using High-performance liquid chromatography (HPLC). The amount of accumulated 24 h CS permeation for all the formulations were observed as 465.95 ± 11.33 µg/cm2 (oleate gel), 33.58 ± 10.30 µg/cm2 (caprylate gel), 32.3 ± 7.83 µg/cm2 (control gel), and 3.48 ± 0.91µg/cm2 (MML). Drug retention in the epidermis skin layer were 13.58 ± 4.36 µg/cm2, 9.01 ± 1.42 µg/cm2, 4.87 ± 1.96 µg/cm2, and 0.44 ± 0.27µg/cm2 for caprylate, oleate, control gel, and MML, respectively. A statistical test was performed using Ordinary one-way ANOVA and the results showed that oleate gel had the highest CS permeation in the receptor compared to all the other formulations (p<0.05). Drug retention in epidermis for oleate was greater than MML (p<0.05) but was no different from caprylate (p>0.05). Caprylate had the highest drug retention in the dermis (106.98 ± 51.44 µg/cm2) compared to oleate (98.24 ± 36.02 µg/cm2), control gel (10.82 ± 1.8 µg/cm2), and MML (4.53 ± 0.35 µg/cm2). Drug content in the dermis was greater from the oleate gel as compared to the control gel and MML (p<0.05). Overall, sodium oleate and sodium caprylate were found to be effective enhancers for topical delivery of cromolyn sodium and the formulated gels worked significantly better than the MML.
Topical Gels for Cromolyn Sodium Delivery in Atopic Dermatitis: Unveiling the Potential of Sodium Salts of Fatty Acids as Permeation Enhancers
D.P. Culp Center Ballroom
Atopic dermatitis, commonly known as eczema, is a chronic inflammatory disorder caused by an overactive immune system. Atopic dermatitis usually develops in early childhood and is prevalent in people with family history of eczema. Several studies have suggested the use of cromolyn sodium (CS), a mast cell stabilizer, as an effective therapeutic agent for treating atopic dermatitis. This study explored the effect of the use of sodium caprylate and sodium oleate as enhancers on topical delivery of CS from a gel-based formulation. An extemporaneously prepared formulation called the Magic Masto Lotion, MML (0.3% CS), has been making waves in terms of the incredible results the lotion offers in the treatment of systemic mastocytosis. Since there is no past literature validating the effectiveness of this MML, another study was established to compare the topical in vitro delivery of CS from MML with the formulated gels. In this study, three separate gels were made by combining CS (4% w/w) with sodium oleate, sodium caprylate and without any enhancer. Drug and enhancers were in the molar ratio of 1:2. Hydroxypropyl cellulose (1.5% w/w) was used as a gelling agent, and respective amounts of propylene glycol as base for the gels. The recipe for the MML was also replicated with CS (0.3% w/w). In vitro permeation studies (24 h) were performed for all formulations using porcine ear skin mounted on Franz diffusion cells maintained at 37°C. Drug content in both the receptor and skin samples were analyzed using High-performance liquid chromatography (HPLC). The amount of accumulated 24 h CS permeation for all the formulations were observed as 465.95 ± 11.33 µg/cm2 (oleate gel), 33.58 ± 10.30 µg/cm2 (caprylate gel), 32.3 ± 7.83 µg/cm2 (control gel), and 3.48 ± 0.91µg/cm2 (MML). Drug retention in the epidermis skin layer were 13.58 ± 4.36 µg/cm2, 9.01 ± 1.42 µg/cm2, 4.87 ± 1.96 µg/cm2, and 0.44 ± 0.27µg/cm2 for caprylate, oleate, control gel, and MML, respectively. A statistical test was performed using Ordinary one-way ANOVA and the results showed that oleate gel had the highest CS permeation in the receptor compared to all the other formulations (p<0.05). Drug retention in epidermis for oleate was greater than MML (p<0.05) but was no different from caprylate (p>0.05). Caprylate had the highest drug retention in the dermis (106.98 ± 51.44 µg/cm2) compared to oleate (98.24 ± 36.02 µg/cm2), control gel (10.82 ± 1.8 µg/cm2), and MML (4.53 ± 0.35 µg/cm2). Drug content in the dermis was greater from the oleate gel as compared to the control gel and MML (p<0.05). Overall, sodium oleate and sodium caprylate were found to be effective enhancers for topical delivery of cromolyn sodium and the formulated gels worked significantly better than the MML.