Unveiling the Importance of SIP68: A UDP Glucosyltransferase on Plant Growth and Immune Responses
Location
D.P. Culp Center Room 303
Start Date
4-5-2024 3:30 PM
End Date
4-5-2024 4:30 PM
Name of Project's Faculty Sponsor
Dhirendra KUMAR
Faculty Sponsor's Department
Biological Sciences
Competition Type
Competitive
Type
Oral Presentation
Presentation Category
Science, Technology and Engineering
Abstract or Artist's Statement
UDP-glucosyltransferases (GTs) are crucial enzymes in plant metabolism, facilitating the transfer of glucosyl groups from UDP-glucose to various acceptor molecules. SIP68, a UDP-glucosyltransferase, has been identified to interact with SABP2 via a yeast two-hybrid screen, and our prior investigations in tobacco plants have underscored its involvement in salicylic acid (SA)-mediated defense signaling. This study aims to elucidate SIP68's potential role in plant development and immune response. Our analysis of SIP68 delves into its gene family, gene and protein sequence, molecular attributes, gene structure, chromosomal localization, exon-intron distribution, cis-regulatory elements in the promoter region, homology modeling, domain architecture, motif analysis, phylogenetic relationships, and protein-protein interactions. Through in-silico examination, we anticipate SIP68's involvement in the cytokinin-mediated metabolic pathway, impacting plant growth and cell proliferation by facilitating the transfer of glucosyl groups to key molecules in this pathway. This suggests SIP68's potential to modulate plant growth and development by influencing the cytokinin pathway. To scrutinize SIP68's role in plant development, we used the SIP68-silenced transgenic tobacco plants. Comparative analysis with wild-type control plants revealed significant alterations in root, shoot, leaf width, and overall biomass development in SIP68-deficient plants, highlighting SIP68's critical role in regulating diverse aspects of plant growth and development. Additionally, SIP68 exhibits a role in response to biotic stresses. To investigate this aspect, tobacco plants were subjected to infection by Tobacco Mosaic Virus (TMV) and bacterial pathogen. This observation underscores that plants with suppressed sip68 expression exhibit reduced resistance when faced with biotic stresses. This finding aligns with our previous observations implicating SIP68 in SA-mediated defense signaling. Further analysis of SIP68's protein-substrate interactions revealed its binding with various flavanols in vitro, offering a platform for exploring potential targets of SIP68 in tobacco plants. Nonetheless, identifying specific in-planta substrates of SIP68 remains a challenge, necessitating continued investigation to unveil its intracellular targets and precise role in plant metabolism. In conclusion, our study provides valuable insights into SIP68's potential contributions to plant development and immune response. The findings underscore SIP68's pivotal role in modulating various facets of plant growth and development. Additionally, our in-silico predictions hint at SIP68's involvement in the cytokinin-mediated metabolic pathway, underscoring its potential impact on plant growth and cell proliferation. Future endeavors are warranted to elucidate SIP68's intracellular targets and its specific role in plant metabolism, emphasizing the significance of UDP-glucosyltransferase enzymes, particularly SIP68, in plant biology.
Unveiling the Importance of SIP68: A UDP Glucosyltransferase on Plant Growth and Immune Responses
D.P. Culp Center Room 303
UDP-glucosyltransferases (GTs) are crucial enzymes in plant metabolism, facilitating the transfer of glucosyl groups from UDP-glucose to various acceptor molecules. SIP68, a UDP-glucosyltransferase, has been identified to interact with SABP2 via a yeast two-hybrid screen, and our prior investigations in tobacco plants have underscored its involvement in salicylic acid (SA)-mediated defense signaling. This study aims to elucidate SIP68's potential role in plant development and immune response. Our analysis of SIP68 delves into its gene family, gene and protein sequence, molecular attributes, gene structure, chromosomal localization, exon-intron distribution, cis-regulatory elements in the promoter region, homology modeling, domain architecture, motif analysis, phylogenetic relationships, and protein-protein interactions. Through in-silico examination, we anticipate SIP68's involvement in the cytokinin-mediated metabolic pathway, impacting plant growth and cell proliferation by facilitating the transfer of glucosyl groups to key molecules in this pathway. This suggests SIP68's potential to modulate plant growth and development by influencing the cytokinin pathway. To scrutinize SIP68's role in plant development, we used the SIP68-silenced transgenic tobacco plants. Comparative analysis with wild-type control plants revealed significant alterations in root, shoot, leaf width, and overall biomass development in SIP68-deficient plants, highlighting SIP68's critical role in regulating diverse aspects of plant growth and development. Additionally, SIP68 exhibits a role in response to biotic stresses. To investigate this aspect, tobacco plants were subjected to infection by Tobacco Mosaic Virus (TMV) and bacterial pathogen. This observation underscores that plants with suppressed sip68 expression exhibit reduced resistance when faced with biotic stresses. This finding aligns with our previous observations implicating SIP68 in SA-mediated defense signaling. Further analysis of SIP68's protein-substrate interactions revealed its binding with various flavanols in vitro, offering a platform for exploring potential targets of SIP68 in tobacco plants. Nonetheless, identifying specific in-planta substrates of SIP68 remains a challenge, necessitating continued investigation to unveil its intracellular targets and precise role in plant metabolism. In conclusion, our study provides valuable insights into SIP68's potential contributions to plant development and immune response. The findings underscore SIP68's pivotal role in modulating various facets of plant growth and development. Additionally, our in-silico predictions hint at SIP68's involvement in the cytokinin-mediated metabolic pathway, underscoring its potential impact on plant growth and cell proliferation. Future endeavors are warranted to elucidate SIP68's intracellular targets and its specific role in plant metabolism, emphasizing the significance of UDP-glucosyltransferase enzymes, particularly SIP68, in plant biology.