Location
D.P. Culp Center Ballroom
Start Date
4-5-2024 9:00 AM
End Date
4-5-2024 11:30 AM
Poster Number
141
Name of Project's Faculty Sponsor
Dhirendra Kumar
Faculty Sponsor's Department
Biological Sciences
Competition Type
Competitive
Type
Poster Presentation
Presentation Category
Science, Technology and Engineering
Abstract or Artist's Statement
In the face of escalating global food security challenges, understanding how plants cope with various environmental stresses such as drought, salinity, and temperature is crucial. In this study, we explored the role of Arabidopsis LTP12 protein in mitigating environmental stresses. To address this, we analyzed the T-DNA knockout mutant ltp12, focusing on its responses to salinity and osmotic stress. Our approach included antioxidant enzyme assays to measure catalase and peroxidase activities, phenotypic analyses assessing water loss, chlorophyll content, seed germination rates, root length, and relative water content, and lipid profile analysis through Thin Layer Chromatography (TLC). Our findings revealed that the mutant ltp12 was compromised in both the catalase and the peroxidase antioxidant enzymatic activities compared to the wild-type Col-0 Arabidopsis plants. The phenotypic analyses further supported these results, showing that compared to the mutant ltp12, the wild-type maintained better hydration, chlorophyll content, seed germination, and growth under stress conditions. Additionally, the lipid profile analysis highlighted an increased presence of phospholipids in the wild-type under high osmotic stress, suggesting a role for LTP12 in lipid reorganization during stress response. The mutant plants also took relatively longer to switch from the vegetative phase to the flowering phase. Collectively, these results underscore the pivotal role of LTP12 protein in enhancing Arabidopsis thaliana's resilience to abiotic stress, paving the way for future research into its structural functions and potential applications in improving crop stress tolerance.
Arabidopsis LTP12 protein has a role in Environmental Stress Response
D.P. Culp Center Ballroom
In the face of escalating global food security challenges, understanding how plants cope with various environmental stresses such as drought, salinity, and temperature is crucial. In this study, we explored the role of Arabidopsis LTP12 protein in mitigating environmental stresses. To address this, we analyzed the T-DNA knockout mutant ltp12, focusing on its responses to salinity and osmotic stress. Our approach included antioxidant enzyme assays to measure catalase and peroxidase activities, phenotypic analyses assessing water loss, chlorophyll content, seed germination rates, root length, and relative water content, and lipid profile analysis through Thin Layer Chromatography (TLC). Our findings revealed that the mutant ltp12 was compromised in both the catalase and the peroxidase antioxidant enzymatic activities compared to the wild-type Col-0 Arabidopsis plants. The phenotypic analyses further supported these results, showing that compared to the mutant ltp12, the wild-type maintained better hydration, chlorophyll content, seed germination, and growth under stress conditions. Additionally, the lipid profile analysis highlighted an increased presence of phospholipids in the wild-type under high osmotic stress, suggesting a role for LTP12 in lipid reorganization during stress response. The mutant plants also took relatively longer to switch from the vegetative phase to the flowering phase. Collectively, these results underscore the pivotal role of LTP12 protein in enhancing Arabidopsis thaliana's resilience to abiotic stress, paving the way for future research into its structural functions and potential applications in improving crop stress tolerance.