Extraction, purification, and characterization of potential bioactive metabolites produced by Janthinobacterium lividum TAJX1901

Authors' Affiliations

Andy Elorm Agbakpo, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Amonah Temitope Arije, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Sean Fox, Department of Health Sciences, College of Public Health, East Tennessee State University, Johnson City, TN. Abbas G. Shilabin, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN.

Location

Culp Center Ballroom

Start Date

4-25-2023 9:00 AM

End Date

4-25-2023 11:00 AM

Poster Number

37

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Abbas Shilabin

Classification of First Author

Graduate Student-Master’s

Competition Type

Competitive

Type

Poster Presentation

Project's Category

Aromatic Compounds, Biochemistry, Organic Chemistry

Abstract or Artist's Statement

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce potent secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel natural compounds. Secondary metabolites from microorganisms have been used in antibiotic production, chemotherapy, immunosuppressants, and various industrial applications. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). To achieve these objectives, the soil bacteria was successfully cultured on rich media agar plates followed by liquid–liquid extraction using a solvent mixture of methanol and chloroform(3:1). Various purification methods were utilized, including flash column chromatography, preparative thin layer chromatography, centrifugation, and high-performance liquid chromatography (HPLC ) using different column types and elution methods. For structural elucidation, UV/Vis analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were employed. The extraction resulted in a dominant violet pigment soluble in methanol. Preliminary results reveal the presence of highly conjugated, polar, and aromatic compounds. This work is relevant in the current global search for novel compounds for tackling antibiotic-resistant organisms and treating other diseases and infections.

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Apr 25th, 9:00 AM Apr 25th, 11:00 AM

Extraction, purification, and characterization of potential bioactive metabolites produced by Janthinobacterium lividum TAJX1901

Culp Center Ballroom

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce potent secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel natural compounds. Secondary metabolites from microorganisms have been used in antibiotic production, chemotherapy, immunosuppressants, and various industrial applications. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). To achieve these objectives, the soil bacteria was successfully cultured on rich media agar plates followed by liquid–liquid extraction using a solvent mixture of methanol and chloroform(3:1). Various purification methods were utilized, including flash column chromatography, preparative thin layer chromatography, centrifugation, and high-performance liquid chromatography (HPLC ) using different column types and elution methods. For structural elucidation, UV/Vis analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were employed. The extraction resulted in a dominant violet pigment soluble in methanol. Preliminary results reveal the presence of highly conjugated, polar, and aromatic compounds. This work is relevant in the current global search for novel compounds for tackling antibiotic-resistant organisms and treating other diseases and infections.