The impact of ovariectomy on the sympathetic response following acute vs prolonged heart failure
Location
Culp Center Ballroom
Start Date
4-25-2023 9:00 AM
End Date
4-25-2023 11:00 AM
Poster Number
129
Faculty Sponsor’s Department
Biological Sciences
Name of Project's Faculty Sponsor
Cerrone Foster
Additional Sponsors
Dr. Krishna Singh
Competition Type
Competitive
Type
Poster Presentation
Project's Category
Cardiovascular Disease
Abstract or Artist's Statement
Currently cardiovascular disease (CVD) is the leading cause of death globally accounting for nearly 17.9 million deaths every year. Studies show that CVD affects men and women differently. A significant increase in CVD incidence is marked by the onset of menopause in women compared to age matched males. A commonality seen in CVD is the use of ꞵ-adrenergic receptors (ꞵ-ARs), as studies have shown that estrogen loss exacerbates the signaling of these ꞵ-AR’s. Changes within this signaling molecule can lead to structural and functional modifications in the heart, including systolic or diastolic dysfunction. This leads to the hypothesis that estrogen loss exacerbates cardiac function with acute sympathetic stimulation and hypertrophy, but prolonged stimulation blunts the sympathetic response. Female mice were ovariectomized (OVX) or underwent SHAM surgery at 2.5 months of age. These mice were treated with isoproterenol (ISO) to simulate chronic sympathetic stimulation for 7, 14, 21 and 28 days continuously through mini osmotic pumps 3 months post ovariectomy. Echocardiography parameters were analyzed using diastolic diameter (DD), systolic diameter (SD), fractional shorting (%FS), and ejection fraction (%EF). Preliminary results showed that %FS and %EF did not change in the OVX and ISO groups compared to SHAM at 7, 14, and 28 days. Similar results were observed in the OVX compared to ISO + OVX groups at 7 days. Even though %FS and %EF did not change, the DD increased at all time points in OVX and ISO groups compared to SHAM. When comparing OVX and ISO + OVX, SD decreased at 14 and 21 days. There is however a significant increase in %FS and %EF at 21 days for all treatment groups. These preliminary results give a better insight to heart function over the course of multiple time points, suggesting that estrogen loss combined with chronic sympathetic stimulation significantly exacerbates the function of the heart.
The impact of ovariectomy on the sympathetic response following acute vs prolonged heart failure
Culp Center Ballroom
Currently cardiovascular disease (CVD) is the leading cause of death globally accounting for nearly 17.9 million deaths every year. Studies show that CVD affects men and women differently. A significant increase in CVD incidence is marked by the onset of menopause in women compared to age matched males. A commonality seen in CVD is the use of ꞵ-adrenergic receptors (ꞵ-ARs), as studies have shown that estrogen loss exacerbates the signaling of these ꞵ-AR’s. Changes within this signaling molecule can lead to structural and functional modifications in the heart, including systolic or diastolic dysfunction. This leads to the hypothesis that estrogen loss exacerbates cardiac function with acute sympathetic stimulation and hypertrophy, but prolonged stimulation blunts the sympathetic response. Female mice were ovariectomized (OVX) or underwent SHAM surgery at 2.5 months of age. These mice were treated with isoproterenol (ISO) to simulate chronic sympathetic stimulation for 7, 14, 21 and 28 days continuously through mini osmotic pumps 3 months post ovariectomy. Echocardiography parameters were analyzed using diastolic diameter (DD), systolic diameter (SD), fractional shorting (%FS), and ejection fraction (%EF). Preliminary results showed that %FS and %EF did not change in the OVX and ISO groups compared to SHAM at 7, 14, and 28 days. Similar results were observed in the OVX compared to ISO + OVX groups at 7 days. Even though %FS and %EF did not change, the DD increased at all time points in OVX and ISO groups compared to SHAM. When comparing OVX and ISO + OVX, SD decreased at 14 and 21 days. There is however a significant increase in %FS and %EF at 21 days for all treatment groups. These preliminary results give a better insight to heart function over the course of multiple time points, suggesting that estrogen loss combined with chronic sympathetic stimulation significantly exacerbates the function of the heart.