Authors' Affiliations

Amonah Arije, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson city, TN. Andy Agbakpo, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson city, TN. Abbas Shilabin, Department of Chemistry, College of Arts and Science, East Tennessee State University, Johnson city, TN. Sean Fox, College of Public Health, East Tennessee State University, Johnson City, TN.

Location

Culp Center Ballroom

Start Date

4-25-2023 9:00 AM

End Date

4-25-2023 11:00 AM

Poster Number

38

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Abbas Shilabin

Classification of First Author

Graduate Student-Master’s

Competition Type

Competitive

Type

Poster Presentation

Project's Category

Biological and Chemical Sciences

Abstract or Artist's Statement

As antimicrobial resistance persistently disrupts the treatment of microbial infection, identifying novel drugs with novel modes of action is critical to getting ahead of resistance. The primary goal of this project is to extract and identify novel chemical products produced by Arthrobacter sp. TAJX1902, particularly antimicrobial metabolites. Although underexplored, Arthrobacter sps. have been shown to produce bioactive compounds of great versatility; one such is a depsipeptide with quorum-sensing inhibitory activity.1 In this research, Arthrobacter sp. TAJX1902 isolated from a soil sample showed inhibitory activity against a filamentous indicator-type bacterium and a violacein-producing Janthinobacterium sp. A. sp. TAJX1902 was cultured using rich medium broth and agar and extracted with solvents of varying polarity. Characterization of purified bioactive compounds from A. sp. TAJX1902 was done via spectroscopic techniques, including 1D and 2D-NMR spectroscopy, FTIR, and GCMS analysis. The A. sp. TAJX1902 was found to produce pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl), and five other bioactive cyclic dipeptides (CDP).

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Apr 25th, 9:00 AM Apr 25th, 11:00 AM

Isolation and Structural Determination of Bioactive Metabolites Produced by a Soil Bacterium, Arthrobacter sp. TAJX1902

Culp Center Ballroom

As antimicrobial resistance persistently disrupts the treatment of microbial infection, identifying novel drugs with novel modes of action is critical to getting ahead of resistance. The primary goal of this project is to extract and identify novel chemical products produced by Arthrobacter sp. TAJX1902, particularly antimicrobial metabolites. Although underexplored, Arthrobacter sps. have been shown to produce bioactive compounds of great versatility; one such is a depsipeptide with quorum-sensing inhibitory activity.1 In this research, Arthrobacter sp. TAJX1902 isolated from a soil sample showed inhibitory activity against a filamentous indicator-type bacterium and a violacein-producing Janthinobacterium sp. A. sp. TAJX1902 was cultured using rich medium broth and agar and extracted with solvents of varying polarity. Characterization of purified bioactive compounds from A. sp. TAJX1902 was done via spectroscopic techniques, including 1D and 2D-NMR spectroscopy, FTIR, and GCMS analysis. The A. sp. TAJX1902 was found to produce pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl), and five other bioactive cyclic dipeptides (CDP).