Extraction and Partial Purification of an Antibiotic-like Compound From the Soil Bacterium Rhoddococcus Strain KCHXC3
Location
Culp Room 210
Start Date
4-6-2022 10:15 AM
End Date
4-6-2022 10:30 AM
Faculty Sponsor’s Department
Chemistry
Name of Project's Faculty Sponsor
Abbas Shilabin
Competition Type
Non-Competitive
Type
Boland Symposium
Project's Category
Chromatography
Abstract or Artist's Statement
Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS were performed in order to determine the purity of this solid.
Extraction and Partial Purification of an Antibiotic-like Compound From the Soil Bacterium Rhoddococcus Strain KCHXC3
Culp Room 210
Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS were performed in order to determine the purity of this solid.