Honors Program
Honors in Chemistry
Date of Award
5-2022
Thesis Professor(s)
Abbas Shilabin
Thesis Professor Department
Chemistry
Thesis Reader(s)
Bert Lampson
Abstract
Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella sonnei. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS revealed the presence of aromatic rings in the active compound that appears to be built with amino acids.
Publisher
East Tennessee State University
Document Type
Honors Thesis - Withheld
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Bond, Elizabeth, "Extraction and partial purification of an antibiotic-like compound from the soil bacterium Rhodococcus strain KCHXC3" (2022). Undergraduate Honors Theses. Paper 713. https://dc.etsu.edu/honors/713
Copyright
Copyright by the authors.