Honors in Chemistry
Date of Award
Thesis Professor Department
Rhodococcus bacteria have many secondary metabolic pathways that may produce novel natural products. The bacterium Rhodococcus strain KCHXC3 was isolated from a soil sample collected near Kingsport, Tennessee and was found to produce an inhibitory compound active against a broad array of bacterial species, including the Gram negative pathogen Shigella sonnei. The aim of this research is to extract and purify the compound for future structure elucidation. A mixture of compounds from 3 month old agar plates inoculated with strain KCHXC3 was extracted using ethyl acetate. The crude extract was then partially purified utilizing a Sephadex LH-20 column, followed by an analytical NH2 HPLC column. This purification resulted in a dried crystalline-like active compound that is white in color and needle-like in shape. Structural studies such as NMR and GC-MS revealed the presence of aromatic rings in the active compound that appears to be built with amino acids.
East Tennessee State University
Honors Thesis - Withheld
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Bond, Elizabeth, "Extraction and partial purification of an antibiotic-like compound from the soil bacterium Rhodococcus strain KCHXC3" (2022). Undergraduate Honors Theses. Paper 713. https://dc.etsu.edu/honors/713
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