Quantitative Determination of D- and L- Enantiomers of Methylphenidate in Brain Tissue by Liquid Chromatography-Mass Spectrometry
Methylphenidate, a psychostimulant used for the treatment of attention deficit hyperactivity disorder and narcolepsy, is administered as a 50:50 racemic mixture, despite the fact that d‐methylphenidate has been shown to have greater pharmacologic activity. This paper presents a validated LC‐MS/MS approach to separation and quantification of methylphenidate enantiomers using a vancomycin column and triethylammonium acetate to enhance the chiral separation. The method is applicable to the monitoring of these enantiomers in mouse brain, with a limit of detection of 0.5 ng/mL and a lower limit of quantification of 7.5 ng/mL.
Combs, Carolyn C.; Hankins, Erin L.; Copeland, Cara L.; Brown, Stacy D.; and Pond, Brooks B.. 2013. Quantitative Determination of D- and L- Enantiomers of Methylphenidate in Brain Tissue by Liquid Chromatography-Mass Spectrometry. Biomedical Chromatography. Vol.27(12). 1587-1589. https://doi.org/10.1002/bmc.2975 ISSN: 0269-3879