Title

2-Deoxyribonolactone Lesions in X-Ray-Irradiated DNA: Quantitative Determination by Catalytic 5-Methylene-2-Furanone Release

Document Type

Article

Publication Date

9-26-2005

Description

(Chemical Equation Presented) Torn genes: DNA tetramers containing the 2-deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X-rays. Upon treatment with spermine as a catalyst, the dL-containing tetramers decompose to 5-methylene-2-furanone (5-MF; see scheme), a characteristic product of dL decomposition. Hence, 5-MP can be used to quantify dL lesions in DNA.

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