2-Deoxyribonolactone Lesions in X-Ray-Irradiated DNA: Quantitative Determination by Catalytic 5-Methylene-2-Furanone Release
(Chemical Equation Presented) Torn genes: DNA tetramers containing the 2-deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X-rays. Upon treatment with spermine as a catalyst, the dL-containing tetramers decompose to 5-methylene-2-furanone (5-MF; see scheme), a characteristic product of dL decomposition. Hence, 5-MP can be used to quantify dL lesions in DNA.
Roginskaya, Marina; Razskazovskiy, Yuriy; and Bernhard, William A.. 2005. 2-Deoxyribonolactone Lesions in X-Ray-Irradiated DNA: Quantitative Determination by Catalytic 5-Methylene-2-Furanone Release. Angewandte Chemie - International Edition. Vol.44(38). 6210-6213. https://doi.org/10.1002/anie.200501956 PMID: 16136620 ISSN: 1433-7851