High-Resolution Imaging of Kidney Vascular Corrosion Casts With Nano-CT
A vascular corrosion cast of an entire mouse kidney was scanned with a modular multiresolution X-ray nanotomography system. Using an isotropic voxel pitch of 0.5 μm, capillary systems such as the vasa recta, peritubular capillaries and glomeruli were clearly resolved. This represents a considerable improvement over corrosion casts scanned with microcomputed tomography systems. The resolving power of this system was clearly demonstrated by the unique observation of a dense, subcapsular mat of capillaries enveloping the entire outer surface of the cortical region. Resolution of glomerular capillaries was comparable to similar models derived from laser scanning confocal microscopy. The high-resolution, large field of view and the three-dimensional nature of the resulting data opens new possibilities for the use of corrosion casting in research.
Wagner, Roger; Van Loo, Denis; Hossler, Fred; Czymmek, Kirk; Pauwels, Elin; and Van Hoorebeke, Luc. 2011. High-Resolution Imaging of Kidney Vascular Corrosion Casts With Nano-CT. Microscopy and Microanalysis. Vol.17(2). 215-219. https://doi.org/10.1017/S1431927610094201 PMID: 21122193 ISSN: 1431-9276