Long Non-Coding RNA Hotairm1 Promotes S100A9 Support of MDSC Expansion during Sepsis
Document Type
Article
Publication Date
1-1-2020
Description
Myeloid-derived suppressor cells (MDSCs) expand during mouse and human sepsis, but the mechanism responsible for this is unclear. We previously reported that nuclear transport of S100A9 protein programs Gr1CD11b myeloid precursors into MDSCs in septic mice. Here, we show that long non-coding RNA Hotairm1 converts MDSCs from an activator to a repressor state. Mechanistically, increased Hotairm1 expression in MDSCs in mice converted S100A9 from a secreted proinflammatory mediator to an immune repressor by binding to and shuttling it from cytosol to nucleus during late sepsis. High Hotairm1 levels were detected in exosomes shed from MDSCs from late septic mice. These exosomes inhibited lipopolysaccharide-stimulated secretion of S100A9 from early sepsis Gr1CD11b cells. Importantly, Hotairm1 knockdown in late sepsis Gr1CD11b MDSCs prevented S100A9 cytosol to nuclear transfer and decreased repression of proimmune T cells. Notably, ectopic expression of Hotairm1 in early sepsis Gr1CD11b cells shuttled S100A9 to the nucleus and promoted the MDSC repressor phenotype. In support of translating the mechanistic concept to human sepsis, we found that Hotairm1 binds S100A9 protein in CD33CD11bHLA-DR MDSCs during established sepsis. Together, these data support that Hotairm1 is a plausible molecular target for treating late sepsis immune suppression in humans and its immune repressor mechanism may be cell autonomous.
Citation Information
Alkhateeb, Tuqa; Bah, Isatou; Kumbhare, Ajinkya; Youssef, Dima; Yao, Zhi Q.; McCall, Charles E.; and Gazzar, Mohamed E., "Long Non-Coding RNA Hotairm1 Promotes S100A9 Support of MDSC Expansion during Sepsis" (2020). ETSU Faculty Works. 215.
https://dc.etsu.edu/etsu-works-2/215