Degree Name

MS (Master of Science)

Program

Mathematical Sciences

Date of Award

12-2004

Committee Chair or Co-Chairs

Don Hong

Committee Members

Anant P. Godbole, Robert B. Gardner, Tiejian Wu

Abstract

Mass Spectrometry, especially matrix assisted laser desorption/ionization (MALDI) time of flight (TOF), is emerging as a leading technique in the proteomics revolution. It can be used to find disease-related protein patterns in mixtures of proteins derived from easily obtained samples. In this paper, a novel algorithm for MALDI-TOF MS data processing is developed. The software design includes the application of splines for data smoothing and baseline correction, wavelets for adaptive denoising, multivariable statistics techniques such as clustering analysis, and signal processing techniques to evaluate the complicated biological signals. A MatLab implementation shows the processing steps consecutively including step-interval unification, adaptive wavelet denoising, baseline correction, normalization, and peak detection and alignment for biomarker discovery.

Document Type

Thesis - unrestricted

Copyright

Copyright by the authors.

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