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Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2026
Committee Chair or Co-Chairs
Ranjan Chakraborty
Committee Members
Dane Scott, Erik Petersen
Abstract
With antibiotic resistance on the rise and developing new antibiotics becoming undesirable, there is a demand for alternatives to traditional antibiotics. To obtain iron and other vital metals in harsh environments, many bacteria produce compounds called siderophores. These compounds use specialized pathways to traverse the cell membrane(s), which can be exploited by attaching antibiotics to slip into the bacteria like a Trojan horse. However, not all siderophores or the bacteria that produce them are equally useful. Therefore, it is important to determine the siderophores produced by novel bacteria, such as those produced by Lelliottia amnigena. To maximize the quantity of siderophores produced by L. amnigena, the media composition and growth conditions were optimized. After extraction and purification using column chromatographic methods, the siderophore was partially chemically characterized using chemical methods including chemical assays, hydrolysis, TLC, ESI-MS and NMR. Based on these results, the produced siderophore was identified as enterobactin.
Document Type
Thesis - restricted
Recommended Citation
Blevins, Noah, "Isolation and Characterization of Siderophore(s) from Lelliottia amnigena" (2026). Electronic Theses and Dissertations. Paper 4742. https://dc.etsu.edu/etd/4742
Copyright
Copyright by the authors.
