Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
5-2025
Committee Chair or Co-Chairs
Marina Roginskaya
Committee Members
Robert Standaert, Gregory Bishop
Abstract
One-electron oxidation plays a major role in oxidative DNA damage in biological cells. Guanine, the most oxidizable DNA base, has been the major focus of studies on oxidative damage to DNA initiated by one-electron oxidation (OEO). The current study aims at understanding the mechanisms of photooxidation of guanine derivatives with OEO in acidic medium. D1A* and D1B*, which are the products of dGuo in acidic medium, were obtained from dGuo upon photolysis at 485 nm of the reaction mixture in the presence of S2O82- as the oxidant and Ru(II)(bpy)32+ as a photosensitizer. D1A* and D1B* were purified by semi-preparative HPLC, and their structures were confirmed by 1H-NMR. Results indicate that the OEO of isolated D1A* and D1B* does not produce D2* in acidic medium and were fairly stable at physiological conditions, which indicates that the structures of D1A*, D1B*, and D2* are different from the structures of D1A, D1B, and D2, respectively.
Document Type
Thesis - unrestricted
Recommended Citation
Ibrahim, Abdul-Kadir, "Effects of pH on Product Yield of One-electron Oxidation to Guanine Derivatives" (2025). Electronic Theses and Dissertations. Paper 4547. https://dc.etsu.edu/etd/4547
Copyright
Copyright by the authors.