Investigating Phytosulfokine Trafficking: Insights into the Role of Phytohormones in Plant Signaling
Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
12-2024
Committee Chair or Co-Chairs
Robert F. Standaert
Committee Members
Catherine McCusker, Aleksey Vasiliev
Abstract
Phytosulfokine is a critical signaling peptide involved in plant growth, development, and stress responses. This thesis investigates the trafficking mechanisms of Phytosulfokine (PSK) in Arabidopsis thaliana, employing fluorescently labelled PSK for non-destructive imaging using a custom-built fiber-optic fluorescence microscope. The study aims to determine the mobility and fate of PSK in plant tissues. Experimental results reveal the short-range and long-range movement of PSK across leaves. Additionally, the project highlights the optimization of non-destructive imaging techniques and the synthesis of caged dexamethasone to facilitate optogenetic studies. These findings provide new insights into PSK trafficking mechanisms in plants, enhancing our understanding of phytohormone-mediated plant stress responses and offering potential agricultural biotechnology applications to improve bioenergy crops.
Document Type
Dissertation - embargo
Recommended Citation
Tindi, Martin, "Investigating Phytosulfokine Trafficking: Insights into the Role of Phytohormones in Plant Signaling" (2024). Electronic Theses and Dissertations. Paper 4485. https://dc.etsu.edu/etd/4485
Copyright
Copyright by the authors.