Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2024
Committee Chair or Co-Chairs
Dr. Ashana Puri
Committee Members
Digavalli, Sivarao Venkata., Schmeichel, Brooke E, Foster Cerrone .R
Abstract
Amiloride, a diuretic used for managing resistant hypertension, is hindered by low oral bioavailability and Gastrointestinal side effects, impacting patient compliance. This study investigates transdermal delivery as an alternative to reduce pill burden and improve adherence. Using dermatomed porcine ear skin, the research examined the passive transdermal delivery of amiloride and the effect of various chemical enhancers, including oleic acid, oleyl alcohol, tocopheryl polyethylene glycol succinate, and N-methyl-2-pyrrolidone, individually or in combination. A validated High-Performance Liquid Chromatography method, adhering to Internal Council for Harmonized guidelines (ICH), was employed for quantitative analysis. In vitro permeation studies, conducted using porcine skin and Franz diffusion cells over 168 hours, revealed that oleyl alcohol (761.86±74.97 µg/cm²) and oleic acid (691.90±78.59 µg/cm²) were the most effective enhancers. These findings suggest that chemical enhancers can significantly facilitate the transdermal delivery of amiloride, offering a promising alternative to enhance patient compliance and achieve better therapeutic outcomes.
Document Type
Thesis - embargo
Recommended Citation
Leshaodo, Oluwatosin Tabitha, "Effect of Chemical Enhancers on Transdermal Delivery of Amiloride for Management of Resistant Hypertension" (2024). Electronic Theses and Dissertations. Paper 4444. https://dc.etsu.edu/etd/4444
Copyright
Copyright by the authors.