Degree Name

MS (Master of Science)

Program

Biology

Date of Award

12-2001

Committee Chair or Co-Chairs

Foster Levy, Elaine Walker

Committee Members

Laraine Powers

Abstract

Antimicrobial susceptibility profiles were assembled for isolates of Moraxella catarrhalis collected from the Mountain Home Veteran's Affairs Medical Center (VAMC) clinical laboratory in Johnson City, Tennessee. The goal of the study was to identify isolates for genetic characterization using comparisons of susceptibility profiles. Isolates of Moraxella catarrhalis collected from July 1984 through 1994 were analyzed for β-lactamase production using a Cefinase disk assay.

A multi-drug profile consisting of 11 β-lactam antibiotics was performed on the 41 M. catarrhalis isolates. Kirby Bauer disk assays were performed for 7 cephalosporin and 4 non-cephalosporin antibiotics.

In summary, 2 observations implicate more complex resistance determinants than the 2 known forms of the BRO β-lactamase. First, there was overlap in the ranges of inhibition zones. Second, several isolates had antibiotic-specific deviations from typical profiles. These data suggest either more variation in the M. catarrhalis BRO β-lactamase than described or contributions to resistance from undescribed determinants.

Document Type

Thesis - unrestricted

Copyright

Copyright by the authors.

Included in

Biology Commons

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