Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2018
Committee Chair or Co-Chairs
Dhirendra Kumar
Committee Members
Ranjan Chakraborty, Gerardo Arcéo-Gomez
Abstract
SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed that SIP470 is localized in the mitochondria. Mitochondria membranes are rich in lipids and have shown lipid exchange with the endoplasmic reticulum in mammalian systems. Co-localization of SIP470-eGFP+mCherry did not express complete co-localization in the targeted organelles. Co-localization pattern suggests possible localization in the endoplasmic reticulum.
Document Type
Thesis - unrestricted
Recommended Citation
Andrews, Shantaya, "Localization of SIP470, a Plant Lipid Transfer Protein in Nicotiana tabacum" (2018). Electronic Theses and Dissertations. Paper 3520. https://dc.etsu.edu/etd/3520
Copyright
Copyright by the authors.
Included in
Biochemistry Commons, Biology Commons, Molecular Biology Commons, Plant Sciences Commons