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Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2016
Committee Chair or Co-Chairs
Aruna Kilaru
Committee Members
Cecilia McIntosh, Cerrone Foster
Abstract
N-acylethanolamines (NAEs) are derived from a minor membrane lipid constituent N-acylphosphatidylethanolamine and are hydrolyzed by fatty acid amide hydrolases (FAAH) into free fatty acid (FFA) and ethanolamine in both plants and animals. In Arabidopsis, NAE plays an important physiological role in growth/development and response to stress. Although NAEs are reported in tomato, their metabolic pathway remains undiscovered. It is hypothesized that there is a functional FAAH in tomato that hydrolyzes NAEs. To this extent, a putative gene that likely encodes for putative SlFAAH1 protein was identified, cloned, and heterologously expressed. Amidase activity was tested using radiolabeled NAE substrates. Furthermore, expression of putative SlFAAH1 transcripts and protein activity was quantified at different developmental stages to demonstrate endogenous amidase activity in tomato seedlings. In future, molecular and biochemical characterization of tomato FAAH will further test the conserved nature of NAE metabolic pathway in plants.
Document Type
Thesis - restricted
Recommended Citation
Tiwari, Vijay, "Isolation and Heterologous Expression of Putative Tomato Fatty Acid Amide Hydrolase" (2016). Electronic Theses and Dissertations. Paper 3138. https://dc.etsu.edu/etd/3138
Copyright
Copyright by the authors.