Degree Name
MS (Master of Science)
Program
Biology
Date of Award
5-2016
Committee Chair or Co-Chairs
Edward M. Onyango
Committee Members
Mohamed Elgazzar, Eric Mustain
Abstract
In the gastrointestinal tract, probiotics have been shown to promote host immunity and to regulate immune signaling pathways. This study used Caco-2 cell line to examine the effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu” a Kenyan traditional cultured milk, on the production inflammatory cytokines in enterocytes. Live Lactobacillus rhamnosus (MRS6AN), its cytoplasmic fraction (CF), filtered spent broth (FSB) or heat inactivated FSB (HIB) were used as treatments on differentiated Caco-2 cell monolayer in transwells. Cytokine content in the cell lysates, apical and basolateral supernatants were determined using ELISA. Caco-2 cell lysate treatments showed significantly increased anti-inflammatory TGF-β (ng/ml) levels on average about 100x more compared to the increase in pro-inflammatory IL-8 (pg/ml) levels. These levels were significantly reduced after inhibition of NF-κB. In conclusion, live Lactobacillus rhamnosus, its CF, FSB or HIB seemed to modulate the production of inflammatory cytokines in enterocytes partly via the NF-κB signaling pathway.
Document Type
Thesis - unrestricted
Recommended Citation
Ngeny, Beverly C., "Effects of Lactobacillus rhamnosus Milk Isolate on the Production of Inflammatory Cytokines in Enterocytes" (2016). Electronic Theses and Dissertations. Paper 3027. https://dc.etsu.edu/etd/3027
Copyright
Copyright by the authors.