Degree Name
MS (Master of Science)
Program
Biology
Date of Award
8-2014
Committee Chair or Co-Chairs
Edward Onyango
Committee Members
Bert Lampson, Victoria Palau
Abstract
Probiotics are viable nonpathogenic microbes that positively affect host health. Probiotics inhibit infection, activate immunity, and promote mucosal-barrier development. Many microbes have probiotic activity. Nonetheless, the selection of stable strains and their specific mechanism(s) of action are not fully elucidated. Bacteria from ‘Amabere amaruranu’ cultured milk from Kenya were isolated and identified by PCR sequence analysis of the 16S rRNA gene. Isolates were examined for stability to acid and bile, antimicrobial activity, mucin production, and degradation and sensitivity to antibiotics, hence their potential for probiotics. Lactobacillus isolates were acid unstable, bile-stable, nonmucinolytic, and presented antibacterial activity. L. rhamnosus cell fractions increased MUC4 and MUC3 expression in colon cells. Bacillus isolates were acid and bile stable, nonmucinolytic and lacked antimicrobial activity. In conclusion, Lactobacillus isolates that were nonmucinolytic, stable in bile, demonstrated antibacterial activity, sensitive to antibiotics, and stimulated increase MUC4 and MUC3 levels in colon cells could be potential probiotics.
Document Type
Thesis - unrestricted
Recommended Citation
Boyiri, Blaise B., "Probiotic Potential of Bacterial Isolates From ‘Amabere amaruranu’ Cultured Milk" (2014). Electronic Theses and Dissertations. Paper 2389. https://dc.etsu.edu/etd/2389
Copyright
Copyright by the authors.