Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
8-2006
Committee Chair or Co-Chairs
Chu-Ngi Ho
Committee Members
Jeffrey G. Wardeska, Ismail O. Kady
Abstract
Cholesterol is an important biological molecule with many important functions. However, high serum cholesterol is a health hazard. Thus analysis of cholesterol is important and many analytical techniques have been developed. The objectives of the proposed research are to develop an economical, rapid method for the determination of total cholesterol with good selectivity and enhanced sensitivity. For evaluation of proposed flow injection analysis with peroxyoxalate chemiluminescence (FIA-POCL) method, figures of merit such as accuracy, precision, and linear dynamic range will be assessed. The proposed procedure was then applied to the determination of total cholesterol in foods. The procedure was linear for cholesterol from 0.01 to 0.120 mg/mL. The relative standard deviation was 2.57%. The recoveries were 97.5-103.3% for commercial standard cholesterol sample, and 101.5-108.0% for butter. The proposed method was applied to analysis of cholesterol in food and the results were consistent with expected values.
Document Type
Thesis - unrestricted
Recommended Citation
Feleke, Abisake, "Determination of Cholesterol in Foods by Flow Injection Analysis with Perroxyoxalate Chemiluminescence." (2006). Electronic Theses and Dissertations. Paper 2231. https://dc.etsu.edu/etd/2231
Copyright
Copyright by the authors.