Degree Name
MS (Master of Science)
Program
Biology
Date of Award
12-2008
Committee Chair or Co-Chairs
Ranjan N. Chakraborty
Committee Members
Dhirena Kumar, Bert C. Lampson
Abstract
Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. Rhizobia are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. R. leguminosarum IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding and transport kinetics of 'schizokinen' and have also attempted to identify the genes involved in its biosynthesis using mini Tn5 random mutagenesis. DNA sequence analysis of a non siderophore producing transconjugant revealed a gene involved in PAC/PAS signal transduction systems that respond to many extracellular cues.
Document Type
Thesis - unrestricted
Recommended Citation
Hammond, David Jack, "Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by Rhizobium leguminosarum IARI 917." (2008). Electronic Theses and Dissertations. Paper 2001. https://dc.etsu.edu/etd/2001
Copyright
Copyright by the authors.