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Degree Name
MS (Master of Science)
Program
Biomedical Sciences
Date of Award
8-2009
Committee Chair or Co-Chairs
James R. Stewart
Committee Members
Rebecca A. Pyles, Thomas W. Ecay Jr.
Abstract
Calbindin-D28K is a cytosolic calcium binding protein found in a variety of cells that transport calcium. The chorioallantoic membrane and yolk sac of oviparous squamate reptiles (lizards and snakes) transport calcium from the eggshell and yolk to the developing embryo. I used immunohistochemistry to localize calbindin-D28K expression in the chorioallantoic membrane and yolk sac of two species of oviparous scincid lizards, Plestiodon fasciatus and Saproscincus mustelinus. Calbindin-D28K was detected in the chorioallantoic membrane and yolk sac of both lizard species by a polyclonal anti-snake calbindin antibody and a monoclonal anti-cow calbindin antibody. Calbindin-D28K was localized in the chorionic epithelium and allantoic epithelium of the chorioallantoic membrane and in endodermal cells scattered throughout the yolk mass of both species. This is the first demonstration of calbindin-D28K in allantoic epithelium and in endodermal cells of the yolk sac of lizards.
Document Type
Thesis - restricted
Recommended Citation
Li, Shuo, "Localization of Calbindin-D28k in Extra-Embryonic Membranes of Two Oviparous Scincid Lizards." (2009). Electronic Theses and Dissertations. Paper 1793. https://dc.etsu.edu/etd/1793
Copyright
Copyright by the authors.