MS (Master of Science)
Date of Award
Committee Chair or Co-Chairs
Alesky Vasiliev, Jeffrey Wardeska
Two batches of CdSe QDs with different sizes were synthesized for immobilizing in polyisoprene (PI), polymethylmethacrylate (PMMA), and low-density polyethylene (LDPE). The combinations of QDs and polymer substrates were evaluated for their analytical fit-for-use in applicable immunoassays. Hydrogen peroxide standards were injected into the flow injection analyzer (FIA) constructed to simulate enzyme-generated hydrogen peroxide reacting with bis-(2,4,6-trichlorophenyl) oxalate.
Linear correlations between hydrogen peroxide and chemilumenscent intensities yielded regression values greater than 0.9750 for hydrogen peroxide concentrations between 1.0 x 10-4 M and 1.0 x 10-1 M. The developed technique’s LOD was approximately 10 ppm. Variability of the prepared QD-polymer products was as low as 3.2% throughout all preparations.Stability of the preparations was tested during a 30-day period that displayed up to a four-fold increase in the first 10 days. The preparations were decently robust to the FIA system demonstrating up to a 15.20% intensity loss after twenty repetitive injections.
Thesis - unrestricted
Moore, Christopher S., "Study of Immobilizing Cadmium Selenide Quantum Dots in Selected Polymers for Application in Peroxyoxalate Chemiluminescence Flow Injection Analysis" (2013). Electronic Theses and Dissertations. Paper 1151. https://dc.etsu.edu/etd/1151
Copyright by the authors.