Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
5-2013
Committee Chair or Co-Chairs
Cassandra Eagle
Committee Members
Alesky Vasiliev, Jeffrey Wardeska
Abstract
Two batches of CdSe QDs with different sizes were synthesized for immobilizing in polyisoprene (PI), polymethylmethacrylate (PMMA), and low-density polyethylene (LDPE). The combinations of QDs and polymer substrates were evaluated for their analytical fit-for-use in applicable immunoassays. Hydrogen peroxide standards were injected into the flow injection analyzer (FIA) constructed to simulate enzyme-generated hydrogen peroxide reacting with bis-(2,4,6-trichlorophenyl) oxalate.
Linear correlations between hydrogen peroxide and chemilumenscent intensities yielded regression values greater than 0.9750 for hydrogen peroxide concentrations between 1.0 x 10-4 M and 1.0 x 10-1 M. The developed technique’s LOD was approximately 10 ppm. Variability of the prepared QD-polymer products was as low as 3.2% throughout all preparations.Stability of the preparations was tested during a 30-day period that displayed up to a four-fold increase in the first 10 days. The preparations were decently robust to the FIA system demonstrating up to a 15.20% intensity loss after twenty repetitive injections.
Document Type
Thesis - unrestricted
Recommended Citation
Moore, Christopher S., "Study of Immobilizing Cadmium Selenide Quantum Dots in Selected Polymers for Application in Peroxyoxalate Chemiluminescence Flow Injection Analysis" (2013). Electronic Theses and Dissertations. Paper 1151. https://dc.etsu.edu/etd/1151
Copyright
Copyright by the authors.
Included in
Analytical Chemistry Commons, Bioimaging and Biomedical Optics Commons, Nanoscience and Nanotechnology Commons