Siderophore Analysis of Soil Isolates
Abstract
Iron is essential for nearly all living organisms, serving as a critical cofactor in metabolic pathways such as the electron transport chain. However, in aerobic and neutral environments, iron is predominantly found as insoluble ferric (Fe3+) hydroxides, limiting its bioavailability. To overcome this, bacteria have evolved to produce siderophores. These are low-molecular weight high-affinity iron chelators that solubilize ferric iron and help facilitate its entry into the bacterial cell via specialized receptors. Siderophores structure is dependent on the bacteria and strain producing them. Conferring a competitive advantage in polymicrobial environments. This has been exploited therapeutically through siderophore-antibiotic conjugates, allowing for targeted drug delivery into pathogenic bacteria. Despite this, many siderophore structures remain uncharacterized. In this study, I sought to purify and characterize a siderophore produced by Pseudomonas mosselii. For this, culture supernatants were subject to affinity chromatography (XAD-4) followed by size-exclusion chromatography (LH-20). Before final purification by reverse-phase C18 HPLC. After purification, mass spectrometry and nuclear magnetic resonance (NMR) will be used for structural elucidation.
Start Time
15-4-2026 9:00 AM
End Time
15-4-2026 10:00 AM
Room Number
303
Presentation Type
Oral Presentation
Presentation Subtype
Grad/Comp Orals
Presentation Category
Science, Technology, and Engineering
Student Type
Graduate
Faculty Mentor
Ranjan Chakraborty
Siderophore Analysis of Soil Isolates
303
Iron is essential for nearly all living organisms, serving as a critical cofactor in metabolic pathways such as the electron transport chain. However, in aerobic and neutral environments, iron is predominantly found as insoluble ferric (Fe3+) hydroxides, limiting its bioavailability. To overcome this, bacteria have evolved to produce siderophores. These are low-molecular weight high-affinity iron chelators that solubilize ferric iron and help facilitate its entry into the bacterial cell via specialized receptors. Siderophores structure is dependent on the bacteria and strain producing them. Conferring a competitive advantage in polymicrobial environments. This has been exploited therapeutically through siderophore-antibiotic conjugates, allowing for targeted drug delivery into pathogenic bacteria. Despite this, many siderophore structures remain uncharacterized. In this study, I sought to purify and characterize a siderophore produced by Pseudomonas mosselii. For this, culture supernatants were subject to affinity chromatography (XAD-4) followed by size-exclusion chromatography (LH-20). Before final purification by reverse-phase C18 HPLC. After purification, mass spectrometry and nuclear magnetic resonance (NMR) will be used for structural elucidation.
