The Effects of Delta-8-tetrahydrocannabinol on Danio rerio

Authors' Affiliations

Dornu Biragbara, Master of Science Biomedical Sciences program, Lincoln Memorial University DCOM, Harrogate, TN Ava Azizi, Master of Science Biomedical Sciences program, Lincoln Memorial University DCOM, Harrogate, TN Lori McGrew, PhD, Department of Pharmocology, Lincoln Memorial University DCOM, Harrogate, TN

Location

Culp Center Ballroom

Start Date

4-25-2023 9:00 AM

End Date

4-25-2023 11:00 AM

Poster Number

61

Faculty Sponsor’s Department

Pharmaceutical Sciences

Name of Project's Faculty Sponsor

Lori McGrew

Classification of First Author

Graduate Student-Master’s

Competition Type

Competitive

Type

Poster Presentation

Project's Category

Healthcare and Medicine

Abstract or Artist's Statement

Delta-8-tetrahydrocannabinol (THC), although less potent than THC, can still reduce memory cognition and anxiety. However, Delta-8 can have adverse effects at high doses, like inducing anxiety, confusion, and hallucinations. The brain of Danio rerio (zebrafish) resembles human neuroanatomical and neurochemical pathways and demonstrates robust behavioral phenotypes, making zebrafish an excellent model organism for memory and anxiety. Subjects will be fed 5mg of Delta 8-THC before entering the experimental apparatus. Fish for both memory and anxiety tests will be observed and recorded through Noldus Ethovision Software. For testing memory, five female and five male zebrafish will be individually placed in a three-compartment memory tank. One side of the memory tank will have a star shape, while the other side will have a circle shape. About .83mg of commercial fish flakes will be administered to the fish on the circle side in the first trial, then on the star side in the second trial, and will continue on either side for 20 trials. Correct responses will be scored when the fish are present on the side of the tank used to present the fish flakes. We hypothesize that there will be fewer correct responses for fish fed with Delta-8-THC than the control. For testing anxiety, ten male and female zebrafish will be fed 5mg of Delta 8-THC before entering the diving tank. Typically, zebrafish experiencing anxiety will stay at the bottom of the diving tank, but if they are relaxed, they will be at the top. We hypothesize that fish fed with Delta-8-THC will spend more time at the top of the diving tank than the control.

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Apr 25th, 9:00 AM Apr 25th, 11:00 AM

The Effects of Delta-8-tetrahydrocannabinol on Danio rerio

Culp Center Ballroom

Delta-8-tetrahydrocannabinol (THC), although less potent than THC, can still reduce memory cognition and anxiety. However, Delta-8 can have adverse effects at high doses, like inducing anxiety, confusion, and hallucinations. The brain of Danio rerio (zebrafish) resembles human neuroanatomical and neurochemical pathways and demonstrates robust behavioral phenotypes, making zebrafish an excellent model organism for memory and anxiety. Subjects will be fed 5mg of Delta 8-THC before entering the experimental apparatus. Fish for both memory and anxiety tests will be observed and recorded through Noldus Ethovision Software. For testing memory, five female and five male zebrafish will be individually placed in a three-compartment memory tank. One side of the memory tank will have a star shape, while the other side will have a circle shape. About .83mg of commercial fish flakes will be administered to the fish on the circle side in the first trial, then on the star side in the second trial, and will continue on either side for 20 trials. Correct responses will be scored when the fish are present on the side of the tank used to present the fish flakes. We hypothesize that there will be fewer correct responses for fish fed with Delta-8-THC than the control. For testing anxiety, ten male and female zebrafish will be fed 5mg of Delta 8-THC before entering the diving tank. Typically, zebrafish experiencing anxiety will stay at the bottom of the diving tank, but if they are relaxed, they will be at the top. We hypothesize that fish fed with Delta-8-THC will spend more time at the top of the diving tank than the control.