Project Title

Qualitative and Quantitative Analysis of Drug Diffusion Into and Across Derma planed Skin

Authors' Affiliations

Dorcas Frempong, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Jagroop Kaur, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Akeemat Tijani, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Sophia Sergent, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Kara Shaw, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Rebecca Lessaint, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Maryam Al Shawi, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Gabrielle Verana, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN Ashana Puri, Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, Johnson City, TN

Location

Culp Ballroom

Start Date

4-7-2022 9:00 AM

End Date

4-7-2022 12:00 PM

Poster Number

30

Faculty Sponsor’s Department

Pharmaceutical Sciences

Name of Project's Faculty Sponsor

Ashana Puri

Classification of First Author

Pharmacy Student

Competition Type

Competitive

Type

Poster Presentation

Project's Category

Musculoskeletal Diseases, Pain, Medical Methodologies or Procedures

Abstract or Artist's Statement

Dermaplaning is a non-surgical cosmeceutical approach gaining popularity for skin rejuvenation. It works to peel off dead layers of skin and re-initiate skin remodeling. The potential of this approach as an active permeation enhancement strategy when used alone or in synergism with other active approaches was envisaged. To date, no study has evaluated the influence of dermaplaning on the transdermal delivery of drugs. In this study we sought to evaluate the influence of this approach on the transdermal permeation of a typical hydrophilic drug, baclofen. The influence of user variation on dermaplaning was evaluated. Moving at an angle of 45° from one end of skin sections < 1mm thick and of area 1 sq.cm, a dermaplane was stroked 4 times across distances of 0.2 inches through the entire skin area. Treated skin were mounted on Franz cells in in vitro diffusion set-ups. Interindividual variability in use and the consequence of this on permeation was assessed by having 3 different individuals work with different samples of skin (n = 4). Skin resistance was determined before and after dermaplaning. The histological evaluation of the dermaplaned skin was done with heamatoxylin and eosin staining of transverse skin sections and compared to the control (no dermaplaning). Methylene blue staining of treated area was done to confirm the removal of stratum corneum (SC). Confocal imaging to validate effect of dermaplane on permeation was also done by evaluating the permeation depth of fluorescein, a hydrophilic dye. A significant drop in electrical resistance post skin treatment with dermaplane strokes was observed for all treatment groups, signifying the depletion of barrier properties of SC. Consequently, significant drug flux and permeation was observed for the model drug, baclofen in 2 h. The average drug flux over 2 h were 159.65 18.90, 213.15 20.19, 169.06 25.06 μg/cm2/h for users 1,2,3, respectively. These were significantly higher compared to the flux for the control (0 μg/cm2/h, p0.05). Histology studies depicted the removal of SC and some parts of viable epidermis. Confocal imaging of the permeation of hydrophylic dye shows deeper diffusion of dermaplaned skin compared to intact control skin. Dermaplaned skin pieces were heavily stained with methylene blue compared to control pieces indicating the loss of SC and exposure of hydrophilic viable epidermis and or dermis to the dye.The results depict the effectiveness of dermaplaning as a transdermal permeation enhancement strategy.

This document is currently not available here.

Share

COinS
 
Apr 7th, 9:00 AM Apr 7th, 12:00 PM

Qualitative and Quantitative Analysis of Drug Diffusion Into and Across Derma planed Skin

Culp Ballroom

Dermaplaning is a non-surgical cosmeceutical approach gaining popularity for skin rejuvenation. It works to peel off dead layers of skin and re-initiate skin remodeling. The potential of this approach as an active permeation enhancement strategy when used alone or in synergism with other active approaches was envisaged. To date, no study has evaluated the influence of dermaplaning on the transdermal delivery of drugs. In this study we sought to evaluate the influence of this approach on the transdermal permeation of a typical hydrophilic drug, baclofen. The influence of user variation on dermaplaning was evaluated. Moving at an angle of 45° from one end of skin sections < 1mm thick and of area 1 sq.cm, a dermaplane was stroked 4 times across distances of 0.2 inches through the entire skin area. Treated skin were mounted on Franz cells in in vitro diffusion set-ups. Interindividual variability in use and the consequence of this on permeation was assessed by having 3 different individuals work with different samples of skin (n = 4). Skin resistance was determined before and after dermaplaning. The histological evaluation of the dermaplaned skin was done with heamatoxylin and eosin staining of transverse skin sections and compared to the control (no dermaplaning). Methylene blue staining of treated area was done to confirm the removal of stratum corneum (SC). Confocal imaging to validate effect of dermaplane on permeation was also done by evaluating the permeation depth of fluorescein, a hydrophilic dye. A significant drop in electrical resistance post skin treatment with dermaplane strokes was observed for all treatment groups, signifying the depletion of barrier properties of SC. Consequently, significant drug flux and permeation was observed for the model drug, baclofen in 2 h. The average drug flux over 2 h were 159.65 18.90, 213.15 20.19, 169.06 25.06 μg/cm2/h for users 1,2,3, respectively. These were significantly higher compared to the flux for the control (0 μg/cm2/h, p0.05). Histology studies depicted the removal of SC and some parts of viable epidermis. Confocal imaging of the permeation of hydrophylic dye shows deeper diffusion of dermaplaned skin compared to intact control skin. Dermaplaned skin pieces were heavily stained with methylene blue compared to control pieces indicating the loss of SC and exposure of hydrophilic viable epidermis and or dermis to the dye.The results depict the effectiveness of dermaplaning as a transdermal permeation enhancement strategy.