Project Title

Purification and Structural Elucidation of Bioactive Metabolites Isolated From Soil Bacterium Arthrobacter Sp. Strain TAJX1902

Authors' Affiliations

Amonah Arije, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Andy Agbakpo, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Sean J. Fox, Department of Biology, College of Arts and Sciences, East Tennessee State University, Johnson City, TN. Abbas G. Shilabin, Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, TN.

Location

Culp Ballroom

Start Date

4-7-2022 9:00 AM

End Date

4-7-2022 12:00 PM

Poster Number

86

Faculty Sponsor’s Department

Chemistry

Name of Project's Faculty Sponsor

Abbas Shilabin

Classification of First Author

Graduate Student-Master’s

Competition Type

Competitive

Type

Poster Presentation

Project's Category

Biological and Chemical Sciences, Biochemistry, Microbiology

Abstract or Artist's Statement

Arthrobacter belongs to an underexplored genus of antimicrobial-producing bacteria. All species in this genus are Gram-positive and commonly isolated from soil and marine sources. This genus is known to exhibit metabolic versatility greatly influenced by environmental conditions and nutrition. Arthrobacter sp. strain TAJX1902 isolated from an unknown soil sample is shown to inhibit a filamentous indicator type bacterium. It produced a few bioactive secondary metabolites in rich medium (RM) broth and agar culture. TAJX1902 crude extract was primarily fractionated using a Sephedex LH-20 column. Further purification achieved via Flash Column Chromatography and Preparative Thin layer Chromatography yielded five prominent crude products. The scope of this project is to fully determine the structure of potential novel antibacterial compounds from TAJX1902 via spectroscopic techniques including full 2D-NMR spectroscopic data set in combination with High-Resolution Mass Spectroscopy (HRMS). The research work is currently underway towards the final purification of the compounds using High-Performance Liquid Chromatography (HPLC) method to enable structural determination and evaluation of their antimicrobial activities.

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Apr 7th, 9:00 AM Apr 7th, 12:00 PM

Purification and Structural Elucidation of Bioactive Metabolites Isolated From Soil Bacterium Arthrobacter Sp. Strain TAJX1902

Culp Ballroom

Arthrobacter belongs to an underexplored genus of antimicrobial-producing bacteria. All species in this genus are Gram-positive and commonly isolated from soil and marine sources. This genus is known to exhibit metabolic versatility greatly influenced by environmental conditions and nutrition. Arthrobacter sp. strain TAJX1902 isolated from an unknown soil sample is shown to inhibit a filamentous indicator type bacterium. It produced a few bioactive secondary metabolites in rich medium (RM) broth and agar culture. TAJX1902 crude extract was primarily fractionated using a Sephedex LH-20 column. Further purification achieved via Flash Column Chromatography and Preparative Thin layer Chromatography yielded five prominent crude products. The scope of this project is to fully determine the structure of potential novel antibacterial compounds from TAJX1902 via spectroscopic techniques including full 2D-NMR spectroscopic data set in combination with High-Resolution Mass Spectroscopy (HRMS). The research work is currently underway towards the final purification of the compounds using High-Performance Liquid Chromatography (HPLC) method to enable structural determination and evaluation of their antimicrobial activities.