Honors Program
Honors in Chemistry
Date of Award
5-2014
Thesis Professor(s)
Dr. Stacy Brown
Thesis Professor Department
Pharmaceutical Sciences
Thesis Reader(s)
Dr. Brooks Pond, Dr. Scott Kirkby
Abstract
In this study, three different chromatographic column chemistries (C18, Pentafluorophenyl (PFP), Hydrophilic Interaction Chromatography (HILIC)) were compared under optimal conditions to determine which stationary performed best in the separation and detection of a mixture of opioids using LC-MS. Furthermore, these stationary phases were examined in three different column technologies – traditional silica, porous shell, and porous polymer (PRP). The PRP column had the best peak shape for all 13 opioids and dominated for later-eluting compounds. In terms of column reproducibility, the Hamilton C18 column had the lowest %RSD values. The Kinetex HILIC produced the most theoretical plates and best resolution for polar compounds as did the Hamilton C18 for nonpolar compounds. Finally, Kinetex PFP and Hamilton PRP both demonstrated themselves as viable alternatives to the C18 column chemistry for analysis of this drug class.
Document Type
Honors Thesis - Open Access
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Recommended Citation
Stallard, Derek, "A systematic column performance comparison for the confirmation of opioids used in pain management by LC-MS" (2014). Undergraduate Honors Theses. Paper 189. https://dc.etsu.edu/honors/189
Copyright
Copyright by the authors.
Included in
Medicinal and Pharmaceutical Chemistry Commons, Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Other Chemicals and Drugs Commons