Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
5-2004
Committee Chair or Co-Chairs
Chu-Ngi Ho
Committee Members
Mian Jiang, Tammy A. Davidson
Abstract
Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
Document Type
Thesis - unrestricted
Recommended Citation
Sexton, Danessa Leann, "Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography." (2004). Electronic Theses and Dissertations. Paper 878. https://dc.etsu.edu/etd/878
Copyright
Copyright by the authors.