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Degree Name
MS (Master of Science)
Program
Chemistry
Date of Award
8-2002
Committee Chair or Co-Chairs
Ismail O. Kady
Committee Members
W. Scott Champney, Jeffrey G. Wardeska, Hamid S. Kasmai
Abstract
In the past two decades, few reports have confirmed the various antibacterial activities of total extract from the seeds of Nigella sativa (Black cumin). In attempts to identify the active ingredients in this extract, the seeds were extracted with hexane. The volatile oil obtained from the crude extract was shown to contain at least three distinct compounds, thymoquinone, p-cymene, and α-pinene, as confirmed by GC/MS and NMR spectroscopy. While p-cymene and α-pinene showed no antibacterial activities, thymoquinone, exhibited remarkable inhibition of the growth of various strains of bacteria. For instance, the IC50 and the Minimal Inhibitory Concentration (MIC) for thymoquinone were found to be 1.31 μg/mL and 3.6 μg/mL respectively, when tested against the gram-positive bacteria, S. aureus. It is also found that thymoquinone significantly inhibites the protein and RNA synthesis in S. aureus.
Document Type
Thesis - restricted
Recommended Citation
Kahsai, Alem Welderufael, "Isolation and Characterization of Active Ingredients from Nigella Sativa for Antibacterial Screening." (2002). Electronic Theses and Dissertations. Paper 692. https://dc.etsu.edu/etd/692
Copyright
Copyright by the authors.